FIG 7.

Relationships between HCoV-NL63 and related viruses at the receptor binding domain. (A) Alignment of NL63-like and 229E-like viruses and related viruses at the receptor binding domain. The positions of three receptor binding motifs (RBMs) are marked with double-arrowed black lines. Residues in the NL63-CoV RBMs that directly contact the ACE2 receptor are marked with red downward arrows. (B) Phylogenetic relationships of NL63-like and 229E-like viruses at the receptor binding domain of HCoV-NL63. The tree is based on an amino acid alignment and midpoint rooted.