FIG 2.

EGFR/PI3K signaling is required for HCMV entry into CD34⁺ HPCs. (A and B) Viral entry assays were performed in CD34⁺ HPCs, as previously described in monocytes and fibroblasts (39, 42, 43). Cells were pretreated with AG1478 (EGFRK inhibitor), LY294002 (PI3K inhibitor), or DMSO solvent control (A) or anti-EGFR blocking antibodies (α-EGFR) or IgG control antibodies (B) before HCMV infection. The cells were infected with HCMV (low-passage-number Towne/E), and following proteinase K treatment, total DNA was isolated to detect viral entry by qPCR amplification of viral DNA corresponding to the UL123 gene region of the genome. The experiment was repeated with two different donors. For all the graphs, the levels of internalized HCMV DNA were normalized to the 4°C binding-only controls and to 18S rRNA as an internal control. Nontemplate controls (NTC) are also shown.