FIG 3.

HCMV trafficking in CD34⁺ HPCs is enhanced by EGFR signaling activated during viral entry. (A) CD34⁺ HPCs were infected with HCMV (TB40-UL32-HCMV/E) and treated with DMSO or AG1478 at 30 mpi. The cells were cytospun onto slides at 1 hpi and 4 hpi and then stained and visualized by confocal microscopy. The cells were stained with anti-gB antibody (Ab) and Alexa Fluor 594-conjugated secondary Ab to detect gB (red). UL32-GFP was stained with an anti-GFP Alexa Fluor 488-labeled antibody (green). DAPI was used to counterstain the nucleus (blue). (B) Cells were infected with HCMV (low-passage-number Towne/E) and stained with specific Alexa Fluor 488-labeled HCMV DNA probe (green) according to the FISH protocol. DAPI was used to counterstain the nucleus (blue). (C) Stained cells (an average of 50 for each experimental group) were analyzed and quantitated to calculate the percentage of total viral DNAs detected in the cytoplasm or nucleus. The means and SEM (error bars) of the results of two independent experiments performed are shown.