FIG 2.

PLVA and PLVB are separated by large genetic distances, and each comprises geographically associated subgroups. (A) Maximum likelihood phylogenetic tree constructed from a 474-bp region of pol. Isolates original to this study are highlighted in bold text. Nodes with <60% bootstrap support have been collapsed. Isolate names provide the following information: (i) PLV clade (clade A or B), (ii) host species (Lru, bobcat; Pco, mountain lion), (iii) animal identification number (Table 3), (iv) sampling location, and (v) sample year (1984 to 2011). Regional PLVA subtrees and pairwise identity matrices demonstrate different patterns of host-virus relationships. Isolates in Florida (B) tend to form well-supported clusters by host species, while most California mountain lion isolates (C) are more closely related to sympatric bobcat isolates than to viruses from other mountain lions.