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. 2017 Feb 14;7:11. doi: 10.1186/s13578-017-0138-6

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Differential expressions of FMRFamide-like peptides before and after parasitism in H. avenae. a Comparison of expression levels of indicated FLPs at pre-parasitic and post-parasitic stages by analyzing transcriptome-sequencing data. * Log₂(fold change) pre/post, normalized Log₂(pre-parasitic RPKM/post-parasitic RPKM), calculated by DEGseq. b Real-time PCR results for the expression of indicated FLPs genes in five different developmental stages. The FLPs expression levels in H. avenae at hatched-J2, parasitic-J2, J3, J4 and female adult stages were normalized to that of egg stage. Statistic significance was determined by one way analysis of variance (ANOVA) test. ***p < 0.001