Table 3.

Putative transcripts with RxLR motif in H. avenae with NR hits (Blastp, E value <1e⁻⁵)

CCN protein	SP sitea	RxLR		Hit accession	Description [species]	E value	Ident (%)	Expression bias
		Start	Motif
ISOTIG15357	27	31	RqLR	KHN74319	Chondroitin proteoglycan 2 [Toxocara canis]	4.00E−11	30	Post
ISOTIG17027	26	39	RsLR	KHN88475	Hypothetical protein Tcan_06414 [Toxocara canis]	2.00E−104	69	–
HOWVQCB02JEWZI_10	22	48	RlLR	KHN81668	Hypothetical protein Tcan_03447 [Toxocara canis]	3.00E−13	36	Pre*
ISOTIG16444	28	53	RlLRQ	CTP81713	BMA-SOL-1 [Brugia malayi]	7.00E−53	46	–
HOWVQCB02JXEXX_14	32	53	RmLR	CCA67693	Hypothetical protein PIIN_01520 [Serendipita indica DSM 11827]	2.00E−24	86	Pre*
ISOTIG15922	24	58	RlLR	AEH42093	Cysteine proteinase 6 [Haemonchus contortus]	1.00E−38	37	Pre
ISOTIG07167	20	59	RlLRD	CDW60295	SPRY domain containing protein [Trichuris trichiura]	3.00E−06	35	–
HOWVQCB02FXQYR_12	33	60	RlLR	KHN81668	Hypothetical protein Tcan_03447 [Toxocara canis]	4.00E−13	34	Pre*
ISOTIG15903	29	63	RaLR	XP_003145427	Leukocyte cell-derived chemotaxin 2 [Loa loa]	3.00E−97	46	–
ISOTIG17370	23	65	RvLR	AAG21334	Hypothetical esophageal gland cell secretory protein 4 [Heterodera glycines]	3.00E−76	47	Post
HPF5SIP01EI8EZ_11	40	69	RmLR	XP_001895511	Histone H2A [Brugia malayi]	8.00E−56	76	Post*
HPF5SIP01BKTZ3_5	36	69	RiLR	XP_002677860	Hypothetical protein NAEGRDRAFT_79489 [Naegleria gruberi]	1.00E−34	49	Post*
ISOTIG10016	28	74	RkLR	KHN78406	Thioredoxin domain-containing protein C06A6.5 [Toxocara canis]	0	64	Post
ISOTIG09138	18	78	RmLRE	EGT56409	CBN-LET-268 protein [Caenorhabditis brenneri]	3.00E−122	45	Post
HO9SJLY04I1AHW_9	39	89	RyLR	KHN81115	La-related protein [Toxocara canis]	3.00E−26	50	Pre*

‘Pre*/post*’ means coding by singleton in pre-/post-parasitic library. The singleton might be the gene with low expression and was sequenced only once

^a SP site means signal peptide cleavage site. NR means non-redundant GenBank proteins database. ‘Pre/post’ means significantly high expression in pre-/post-parasitic stage