Table 4.

Transcripts specifically highly expressed at H. avenae parasitic stages (except adult female) with NR hits (Blastx, E value <1e⁻⁵)

CCN contig	Log2(FC)	Q value	Hit accession	Description [species]	E value	Ident (%)
isotig13402	6.89	3.35E−134	YP_009029999	NS5-like protein [Jingmen tick virus]	3.00E−91	31
isotig13435	6.53	1.72E−127	ACF19855	ANT-34 [Toxocara canis]	6.00E−48	32
isotig14470	5.78	1.09E−07	WP_014934453	50S ribosomal protein L25/general stress protein Ctc [Cardinium endosymbiont of Encarsia pergandiella]	1.00E−86	74
isotig12660	5.12	2.24E−05	WP_068700363	Glycoside hydrolase [Paenibacillus yonginensis]	1.00E−26	45
isotig15541	4.98	1.88E−08	EMG48930	Hypothetical protein G210_0425 [Candida maltosa Xu316]	0.003	29
isotig17002	4.94	3.26E−08	WP_011271320	Heat-shock protein C2 [Rickettsia felis]	1.00E−41	55
isotig19536	4.94	6.67E−05	WP_034577564	Preprotein translocase subunit SecE [Cardinium endosymbiont of Bemisia tabaci]	8.00E−25	77
isotig19691	4.84	1.15E−04	XP_002672706	Predicted protein [Naegleria gruberi]	5.00E−33	48
isotig15266a	4.73	1.99E−04	XP_015393075	PREDICTED: cuticle protein 12.5-like [Panthera tigris altaica]	1.00E−16	41
isotig18086	4.49	6.12E−04	ACF19853	ANT-5 [Toxocara canis]	1.00E−38	51
isotig20036	4.49	6.12E−04	KNA02486	Hypothetical protein PVNG_03779 [Plasmodium vivax North Korean]	5.00E−12	77
isotig14974	3.61	7.60E−04	WP_034577458	Transcription termination/antitermination protein NusA [Cardinium endosymbiont of Bemisia tabaci]	0	76
isotig16565	2.92	2.52E−07	KFH65214	Hypothetical protein MVEG_08695 [Mortierella verticillata NRRL 6337]	1.00E−22	45
isotig16605	2.73	2.34E−05	WP_014934894	50S ribosomal protein L20 [Cardinium endosymbiont of Encarsia pergandiella]	1.00E−55	87
isotig14490	2.54	5.90E−07	XP_005705008	DNA-directed RNA polymerase subunit alpha [Galdieria sulphuraria]	4.00E−58	45
isotig16931	1.72	5.88E−04	WP_014934892	Translation initiation factor IF-3 [Cardinium endosymbiont of Encarsia pergandiella]	4.00E−82	77

^a With signal peptides. Log₂ (FC) = normalized Log₂(post-parasitic RPKM/pre-parasitic RPKM). Both Log₂ (FC) and Q value threshold (Storey et al. [44]) were calculated by DEGseq. NR means non-redundant GenBank proteins database