Fig. 2.

Multiple sequence alignment of the hinge and reactive center loop regions of P. xylostella serpins. The hinge and RCL regions of P. xylostella serpins were aligned with those of serpins with known functions from D. melanogaster (Dm), M. sexta (Ms) and A. gambiae (Ag) by ClustalX 2.0 program with default parameters. Predicted P1 residues are highlighted in red. P. xylostella serpins are presented in numerical order and grouped with homologous serpins from other species as determined by phylogenetic analysis (Fig. 3)