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. 2017 Feb 14;17:110. doi: 10.1186/s12906-017-1620-8

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — HPLC profiles of 50% ethanolic extract prepared from the fruits of T. chebula, chebulagic acid and chebulinic acid. The crude extract prepared from the fruits of T. chebula (100 μg), chebulagic acid (20 μg) and chebulinic acid (20 μg) were resolved by reverse phase HPLC using RP 18 column as described in Methods. The X-axis represents the time (min) and Y-axis represents the voltage (mAU) at 272 nm. Solvent used was acetonitrile : H₂O (20 : 80 v/v) supplemented with 0.01% orthophosphoric acid. a 50% ethanolic extract prepared from the fruits of T. chebula, b chebulagic acid and (c) chebulinic acid. The peak corresponding to gallic acid in panel (a) was identified by running gallic acid standard (data not shown)