Fig. 2.

Efficacy of the T. chebula extract, chebulagic acid, chebulinic acid and acyclovir to inhibit attachment of HSV-2 to Vero cells. HSV-2 (100 PFU) virus along with various concentrations of the T. chebula extract, chebulagic acid, chebulinic acid and acyclovir was incubated for 3 h with the pre-chilled Vero cells seeded in 24-well plates at 4 °C and processed for inhibition in the attachment of the virus as described in Methods. The Y-axis represents percent inhibition in the number of plaques with respect to the vehicle treated virus control group. Values are expressed as mean ± SEM of the three independent experiments performed in duplicate. Various panels are (a) extract of T. chebula, b chebulagic acid, c chebulinic acid and (d) acyclovir. ^* p ≤ 0.05, ^** p ≤ 0.01, and ^*** p ≤ 0.001 between treated and vehicle control at respective concentration of the extract/compound