Predicted fish counts (based on modelling performed by Rey et al. [1] in their electronic supplementary material, fig. S2) in each chamber at time 60 min for control and experimental conditions. The column ‘difference in fish count’ represents the change in predicted fish counts in each chamber between experimental and control conditions. Italicized rows are hyperthermic chambers. There is only a modest total increase in predicted fish count of 1.13 (out of a total of approximately 12 fish) in the experimental compared to control hyperthermic chambers. We converted control and experimental fractional values to integers and found no statistical difference between predicted counts in the hyperthermic chambers versus all other chambers (using Fisher's exact test with either one- or two-tailed p-values; p < 0.05). Hyperthermic chambers in the experimental condition would require a predicted fish count of eight (rather than the current four) to reach statistical significance (p < 0.05).