Fig. 2.

RhoA activation is required for Wnt5a-induced cell migration. Cell migration rate was determined by using Boyden chamber assays in MG-63 and U20S cells incubated in the absence (0 ng/mL) or presence of 100 ng/mL Wnt5a for 4 h. a, d MG-63 (a) and U20S cells (d) were transfected with RhoA-siRNA or scrambled siRNA (NC), then subjected to Boyden chamber assays. b, e MG-63 (b) and U20S cells (e) were transfected with GFP-RhoA-N19 (DN-RhoA) or vector, then subjected to Boyden chamber assays. c, e MG-63 (c) and U20S cells (e) were transfected with GFP-RhoA-V14 (CA-RhoA) or vector, then subjected to Boyden chamber assays. All data were presented as mean ± SD of 3 determinations