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. 2017 Feb 14;17:27. doi: 10.1186/s12935-017-0396-8

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — RhoA acts as the downstream of Wnt5a/PI3K/Akt signaling. a Human osteosarcoma cells MG-63 were transfected with RhoA-siRNA or scrambled siRNA (Ctrl) for 48 h, then were treated with 100 ng/mL of Wnt5a and harvested at 30 min after the start of Wnt5a treatment for SDS-PAGE and immunoblot analysis with antibodies to p-Akt and total Akt. p-Akt/total Akt ratios were normalized to the average value of Ctrl group. Data were presented as mean ± SD of 3 determinations. b, c MG-63 (b) and U20S cells (c) were transfected with DN-RhoA (GFP-RhoA-N19) or vector (Ctrl) for 48 h, then were treated with 100 ng/mL of Wnt5a and harvested at 30 min after the start of Wnt5a treatment for SDS-PAGE and immunoblot analysis. p-Akt/total Akt ratios were normalized to the average value of Ctrl group. Data were presented as mean ± SD of 3 determinations. d A schematic illustration of the regulation of RhoA in Wnt5a/PI3K/Akt signaling (See text for detail)