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. 2017 Feb 15;18:16. doi: 10.1186/s12863-017-0481-y

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — tvhydii1 knockout and overexpressing strains. a confirmation of the tvhydii1 gene deletion; lane 1, 5’ end of the deletion cassette inserted at the tvhydii1 locus; lane 2, tvhydii1 wild type amplification as control; lane 3, tvhydii1 amplification in the knockout strains; lane 4, 3’ end of the deletion cassette inserted at the tvhydii1 locus. b semi-quantitative RT-PCR of the knockout and overexpressing strains; RT-PCR reactions were carried out using gpd gene as amplification control for tvhydii1 gene; c hydrophobicity assay, a 20 μL drop of distilled water was placed over plugs of 72 h mycelia of each T. virens strain