Fig. 4.
Total and phosphorylated Smad3 localisation in mouse StHdh cell lines. Fixed StHdhQ7/7, StHdhQ7/111 and StHdhQ111/111 cells stimulated with either 0 or 100 ng/ml TGFβ1 for 30 min, stained with antibodies against A. Smad3 and B. phosphorylated Smad3, with nuclei counterstained with DAPI (blue). Images are representative of multiple replications. Scale bar = 20 μM. Figure representative of multiple images, N = 3. C. Quantification of the nuclear/cytoplasmic mean pixel intensity (N/C MPI) ratio of the experiments presented in A and B, based on an average of 90 cells per condition, taken from 6 different frames across 3 experiments (see Section 2, Methods). Asterisks denote a significant difference from 0 ng/ml TGFβ, hashes indicate a significant difference from StHdHQ7/7 cells. */# p < 0.05, **/## p < 0.01, ***/### p < 0.001.