Figure 1.

CD4⁺ and CD8⁺ T-cell numbers in the thymus are unaffected by PI expression. Live single-positive CD4⁺ and CD8⁺ T cells were quantified in single-cell suspensions of thymus from mice aged 4–7 or 12–16 weeks. Representative flow cytometry plots (A), total CD4⁺ T-cell numbers (B), and total CD8⁺ T-cell numbers (C) are displayed for all strains. Data are shown for 9–12 mice per group (encompassing three to four experiments). Males and females were included because no differences were detected when analyzed separately. **P < 0.01.