Table 1.
Transgenic and knockout mice used in this study
| Genotype | TCRα-chain | TCRβ chain | PI1 expression | PI2 expression | Diabetes |
|---|---|---|---|---|---|
| A22Cα−/− | G9 TCRα transgene (insulin B15-23–specific) | Endogenous TCRβ chains | Normal | Normal | No diabetes |
| A22Cα−/−PI2tg | G9 TCRα transgene (insulin B15-23–specific) | Endogenous TCRβ chains | Normal | Overexpression (under MHC class II promoter) | No diabetes |
| A22Cα−/−PI1−/− | G9 TCRα transgene (insulin B15-23–specific) | Endogenous TCRβ chains | Deficient | Normal | No diabetes |
| A22Cα−/−PI2−/− | G9 TCRα transgene (insulin B15-23–specific) | Endogenous TCRβ chains | Normal | Deficient | Accelerated diabetes in males |
| A22Cα−/−PI1−/−PI2−/−Y16Atg | G9 TCRα transgene (insulin B15-23–specific) | Endogenous TCRβ chains | Lack both native PI genes but express a mutated insulin transgene | No diabetes | |