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. Author manuscript; available in PMC: 2017 Feb 15.
Published in final edited form as: Langmuir. 2016 Jan 4;32(2):551–559. doi: 10.1021/acs.langmuir.5b03445

Figure 6.

Figure 6

Affinity capture of MSP nanodisc containing purified His-MalFGK2. (A) Negative stain TEM of stabilized 100% mPEG350-DTPE monolayer-coated grid after treatment with His-MalFGK2 in nanodiscs; (B) negative stain TEM of His-MalFKG2 in nanodisc captured on stabilized 1:99 Ni2+:NTA-PEG2000-DSPE:mPEG350-DTPE monolayer-coated grid; (C) same as in (B), except that the grid was rinsed with 500 mM imidazole, pH = 7.4 after the His-MalFGK2 in nanodisc exposure step; and (D) cryoEM of His-MalFGK2 in nanodisc captured on stabilized 5:95 Ni2+:NTA-PEG2000-DSPE:mPEG-350-DTPE monolayer-coated grid.