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. 2016 Dec 18;245(3):641–657. doi: 10.1007/s00425-016-2632-1

Table 3.

PSI and PSII electron transport activity in isolated mesophyll (M) and bundle sheath (BS) thylakoids of Echinochloa crus-galli, Digitaria sanguinalis, and Zea mays chloroplasts from control plants (200 µmol photons m−2 s−1) and then transferred to high light (HL) for 1 h (1600 μmol photons m−2 s−1)

Light intensity (µmol photons m−2 s−1) PSII activity (µmol DCPIP mg−1 Chl h−1) PSI activity (µmol O2 mg−1 Chl h−1)
200 1600 200 1600
M BS M BS M BS M BS
Zea mays 89 ± 1 15 ± 2 47 ± 1 10 ± 1 296 ± 1 1020 ± 9 156 ± 2 565 ± 3
Digitaria sanguinalis 40 ± 1 32 ± 3 37 ± 1 25 ± 9 284 ± 6 657 ± 1 176 ± 1 407 ± 4
Echinochloa crus-galli 57 ± 2 30 ± 7 49 ± 4 22 ± 9 189 ± 2 671 ± 2 156 ± 9 429 ± 5

Details are given in Materials and methods. The electron transport activities of PSI were measured polarographically at 25 °C. Chloroplast equivalents of 30 µg mL−1 (M) and 10 µg mL−1 (BS) of chlorophyll were used for PSI measurements, chloroplast equivalents of 20 µg mL−1 of chlorophyll were used for PSII measurements. DCPIP was 0.1 mM. Values are mean ± SD of three independent experiments for PSI, means of 4–5 separate experiments ± SE (three replicates each) for PSII