Fig. 1.

Analysis of the nuclear localization and translocation of TRAFs 1–7 in Neuro2a cells following CD40L stimulation. a Western blot analysis of the subcellular localization of TRAFs 1–7 in Neuro2a cells, as well as the effect of CD40L stimulation on the subcellular localization of TRAFs 1–7. Neuro2a cells were either untreated (control) or stimulated for 5, 10, or 30 min with mouse CD40L (100 ng/mL), cytoplasmic and nuclear extracts were then prepared, and a Western blot performed. As controls for both the loading and the relative purity of the cytoplasmic and nuclear extracts, Glut3 was used as a cytoplasmic marker, and RNA pol II was used as a nuclear marker. b Confocal images of TRAF2 and TRAF3 in Neuro2a cells showing subnuclear puncta