Fig. 2.

Analyses of the nuclear translocation of TRAF2, TRAF3, and RelA in Neuro2a cells, cortical neurons, and Daudi cells. a Western blot analysis of the effect of CD40L stimulation on the subcellular localization of TRAFs 2 and 3 in Neuro2a cells. Neuro2a cells were either untreated (control) or stimulated for 5, 10, or 30 min with mouse CD40L (100 ng/mL), cytoplasmic and nuclear extracts were then prepared, and a Western blot performed. As controls for both the loading and the relative purity of the cytoplasmic and nuclear extracts, HSP60 was used as a cytoplasmic marker, and Lamin A/C was used as a nuclear marker. b Western blot analysis of the effect of CD40L stimulation on the subcellular localization of TRAFs 2 and 3 in primary cortical neurons. E15 mouse cortical neurons were either untreated (control) or stimulated for 5, 10, or 30 minutes with mouse CD40L (100 ng/mL), cytoplasmic and nuclear extracts were then prepared, and a Western blot performed. As controls, Glut3 was used as a cytoplasmic marker and HDAC1 was used as a nuclear marker. c Western blot analysis of the effect of CD40L stimulation on the subcellular localization of TRAFs 2 and 3 in Daudi cells. Daudi cells were either untreated (control) or stimulated for 5, 10, or 30 min with human CD40L (100 ng/mL), cytoplasmic and nuclear extracts were then prepared, and a Western blot was performed. HSP60 and HDAC1 were used as controls. C control