Fig. 11.

RT-qPCR analysis of gene expression changes induced by the transgenic expression of Isl1 in the cerebellum. a Representative 2 % agarose gel electrophoresis of RT-qPCR products shows the expression of Isl1 in Tg ^+/− cerebella of 1-, 7-, and 11-month-old mice (two samples/genotype/age). Hprt1 was used as the reference gene. Lane: PC, positive control (hindbrain); NC, negative control (H₂O). b The expression of genes was analyzed in WT and Tg ^+/− cerebella from 1-month-old mice; the relative expression levels were quantified using −ΔΔCq method. The data represent the expression of mRNA relative to the control cerebella, normalized by the reference gene Hprt1. *P < 0.05; **P < 0.01, t test. The values are means ± SEM (each experiment in duplicate; N = 8/group). Dlg4, discs large homolog 4; Cacng1, voltage-dependent calcium channel gamma subunit 1; Cr, calretinin