Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Feb 3;54(2):1352–1368. doi: 10.1007/s12035-016-9716-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2016

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

PMC Copyright notice

Fig. 4 — Changes in the cerebellum. a, b P3 sagittal sections using Hoechst nuclear staining show the different organizations in the control (WT) and mutant (Tg ^+/−) littermate cerebellar foliation (insert a, b) and disorganization of lobule I + II. Note absence of a recognizable anterior medullary velum (AMV) and the rostral expansion of a hemilobe only in the transgenic mouse (arrowhead). c, d Pax2 (red) and calbindin (green; Purkinje cells) staining of sagittal sections of the anterior lobe of the cerebellar vermis at P3 shows a comparable distribution of Purkinje cells and Pax2⁺ cells in WT (C) and Tg ^+/− lobules (d). The altered foliation of lobules I–III is obvious in the Tg ^+/− cerebellum. e, f Hematoxylin-eosin staining of the brain sections at the level of vermis at P15. The predominant phenotype of altered formation of vermis lobules leading to the fusion of I–III and a hemilobule on top of or as part of the anterior medullary velum (arrow) is detected in the Tg ^+/− cerebellum. The remnant of the inferior colliculus (IC) is denoted by a red asterisk in the Tg ^+/− midbrain. The superior colliculus (SC) and IC are outlined by blue- and red-dashed lines, respectively. g, h The adult Tg ^+/− cerebellum shows the defect in the foliation of the anterior lobe compared to WT littermates as shown by Hoechst staining of the granule cell layer nuclei. The fissure (*) between anterior folia I/II and III failed to form properly, leading to the fusion of the lobules. A hemilobule is on top of or as part of the anterior medullary velum (arrow). The lobules IV–V in Tg ^+/− differ from controls. Roman numerals depict cerebellum lobules. AMV, anterior medullary velum; Calb, calbindin; EGL, external granule layer; IGL, internal granule layer; IC, inferior colliculus; ML, molecular layer; SC, superior colliculus. Scale bar 100 μm (a–d) and 1000 μm (e–h)