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. 2016 Feb 3;54(2):1352–1368. doi: 10.1007/s12035-016-9716-6

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© The Author(s) 2016

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 9 — Altered distribution of calretinin-labelled cells in lobules X and IX of the transgenic cerebellum. Calretinin⁺ cells are primarily found in lobules X and half of IX as shown by calretinin staining (red) in both WT (a) and Tg ^+/− (b) cerebella. Double staining with anti-Calbindin (Calb, green) and anti-Calretinin (red) and visualization of nuclei with Hoechst staining of 100 μm sections of P16 cerebella. Scale bar 500 μm. Quantification of calretinin staining in lobules IX and X of the cerebellum at P16 (c) and 8-month-old (d) using ImageJ. The values represent an average percentage of calretinin⁺ area/lobule area ± SEM (n = 6 Tg ^+/− and 6 WT/each age group), t test *, P < 0.05