Figure 1.

Localities of specimens and their COI barcodes. Localities are shown for all barcoded specimens of C. cecrops (circles) and C. isobeon (squares), and specimens of C. quintana and C. bactra with complete genomes (star, the same locality for both). Localities where specimens with atypical barcodes (for cecrops/isobeon) were present are shown in red. A distance dendrogram built from the barcodes is shown in the middle, and alignment of barcodes with invariant positions removed is on the right. The most frequent nucleotides at each position are not shaded, next most frequent is shaded red, and the third is shaded green. Positions are numbered above the alignment. The last line indicates position in a codon. Protein sequence with invariant positions removed is shown to the right after ‘|’. Specimens with ‘NVG-’ numbers are sequenced in this study and GenBank accession is given for others. Specimens with complete genomes are marked with a dot to the right of the name and a number (1 to 27) that points to locality of the specimen on the map. (Online version in colour.)