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. 2017 Feb 9;12:1143–1160. doi: 10.2147/IJN.S121299

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© 2017 Bacakova et al. This work is published and licensed by Dove Medical Press Limited

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Immunofluorescence staining of protein nanocoating on membrane.

Notes: Fibrin nanocoating (A), collagen nanocoating (D), fibronectin on fibrin nanocoating (B), fibronectin on collagen nanocoating (E), freshly deposited on poly(lactide-co-glycolic acid) membranes. Image A was merged with B (C), and image D with E (F). Secondary antibodies were conjugated with Alexa 488 (fibrin, collagen, green fluorescence) or with Alexa 633 (fibronectin, red fluorescence). Leica TCS SPE DM2500 confocal microscope, magnification 40×/1.15 NA oil.