Table 2.
Antigens that discriminate between A and C/M T. gondii infections. Antigens are ranked by descending protein array signal intensity (see Fig. 3 for details). AUC, sensitivity and specificity refer to detection of C/M or A infections using the C/M-associated or A-associated antigens, respectively. pBH, Benjamini–Hochberg corrected P value; SI ratio, ratio of average signal intensity of (C/M)/A for the C/M-associated antigens, and A/(C/M) for the A-associated antigens; AUC, area under ROC curve; * antigens selected for scale-up purification and analysis by ELISA
| Rank | Antigen (gene ID_exon) | Description | pBH | SI ratio | AUC | Sensitivity (%) | Specificity (%) | |
|---|---|---|---|---|---|---|---|---|
|
| ||||||||
| Original nomenclature | New nomenclature | |||||||
| C/M-associated | ||||||||
| 1* | 067680_8 | 267680_8 | Microneme protein 12 (MIC12) | 0·00015 | 4·9 | 0·818 | 78·1 | 87·1 |
| 2 | 008370_4 | 208370_4 | Myosin heavy chain, putative | 0·00339 | 19·1 | 0·721 | 56·3 | 96·8 |
| 3 | 003290_1 | 203290_1 | Hypothetical protein | 0·00206 | 180·3 | 0·780 | 62·5 | 90·3 |
| 4 | 111720_5 | 311720_5 | Chaperonin protein | 0·04029 | 40·5 | 0·602 | 43·8 | 100·0 |
| 5 | 015780_1 | 215775_1 | Rhoptry protein 8 (ROP8) | 0·00001 | 10·7 | 0·859 | 75·0 | 96·8 |
| 6 | 077240_1 | 277240_1 | NTPase I | 0·00001 | 89·2 | 0·873 | 84·4 | 83·9 |
| A-associated | ||||||||
| 1 | 001780_2 | 201780_2 | Microneme protein 2 (MIC2) | 0·00001 | 2·3 | 0·844 | 90·3 | 81·3 |
| 2 | 033450_1 | 233450_1 | SAG-related sequence 29A (SRS29A) | 0·03802 | 1·4 | 0·691 | 100·0 | 40·6 |
| 3 | 047000_1 | 247000_1 | Tetratricopeptide repeat-containing protein | 0·00011 | 3·4 | 0·858 | 87·1 | 81·3 |
| 4 | 088960_2Seg1 | 288960_2Seg1 | Hypothetical protein | 0·00034 | 3·2 | 0·831 | 77·4 | 81·3 |
| 5* | 095700_6 | 295710_6 | HECT-domain (ubi-quitin-transferase) domain-containing protein | 1·01E-09 | 4·8 | 0·894 | 100·0 | 78·1 |
| 6 | 020950_1 | 220950_1 | Hypothetical protein | 0·00166 | 1·6 | 0·805 | 90·3 | 71·9 |
| 7 | 033530_1 | 2335301 | Hypothetical protein | 0·00001 | 3·1 | 0·847 | 100·0 | 68·8 |
| 8* | 039440_1 | 239440_1 | Protein kinase (incomplete catalytic triad) | 0·00336 | 5·0 | 0·803 | 83·9 | 78·1 |
| 9 | 058950_2 | 258950_2 | Lectin family protein | 0·02013 | 1·6 | 0·770 | 87·1 | 65·6 |
| 10 * | 011460_1 | 211460_1 | Hypothetical protein | 0·00010 | 54·9 | 0·847 | 64·5 | 90·6 |
| 11 | 118460_1 | 211460_1 | Hypothetical protein | 0·04892 | 2·7 | 0·752 | 93·5 | 53·1 |
| 12 | 055180_7 | 255180_7 | Ubiquitin carboxyl-terminal hydrolase | 0·02849 | 4·0 | 0·746 | 64·5 | 81·3 |
| 13 | 053700_4 | 253700_4 | Transporter, major facilitator family protein | 0·00115 | 4·1 | 0·818 | 90·3 | 59·4 |
| 14 | 101270_4 | 301270_4 | Tyrosine kinase-like (TKL) protein | 0·00094 | 11·2 | 0·837 | 83·9 | 75·0 |
| 15 | 048540_12 | 248540_12 | Hypothetical protein | 0·05629 | 13·5 | 0·716 | 74·2 | 59·4 |
| 16 | 008560_1 | 208560_1 | Carrier superfamily protein | 0·00232 | 4·4 | 0·797 | 77·4 | 71·9 |