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. Author manuscript; available in PMC: 2017 Feb 15.
Published in final edited form as: J Bone Miner Res. 2015 Aug 6;31(1):163–172. doi: 10.1002/jbmr.2590

Figure 2. Histology of Decalcified Femur Specimen.

Figure 2

(A) A low-power image of the H&E stain shows dense bone from cortex to cortex (top to bottom).

(B) Polarized image of picrosirius red stain in the center of the specimen demonstrates lamellar bone (appears solid red) admixed with woven bone (hatched red).

(C) A slightly magnified view of the area in B, stained with H&E, shows an irregular arrangement of osteocytes, with the more tightly grouped cells corresponding to areas of woven bone, and sparsely cellular areas with lamellar bone.

(D) Toluidine stained section near the periosteal surface (just out of the field to the right) shows some retained cartilaginous matrix (purple).

(E) Numerous morphologically normal osteoclasts are identified in the specimen on H&E stains, associated with scalloped bone surfaces indicative of active resorption.

(F) The small amount of bone marrow in the specimen shows normal hematopoietic elements, without marrow fibrosis.

Scale bars: A = 2 mm; B,D = 500 μm; C,F = 200 μm; E = 100 μm.