Table 3. The 12 microsatellite markers used in the present study.

Microsatellite marker	Forward (F) and reverse (R) primer sequence (5’-3’)	Primer concentration (μM)	Allele frequency differential (δ)
Panel 1
D15	F: [VIC] 5’–GCTCTCTGAAGAGGCCCTG– 3’ R: 5’–ATTCCAAGAGTGACAGCATCC– 3’	0.200.20	0.62
EF15-Mluc	F: [PET] 5’–GATCGCAGTCCCTTCC– 3’ R: 5’–GCTTATGGGGAGAAATGAG– 3’	0.270.27	-
F19	F: [VIC] 5’–GCTAGCCATGGAGAAGGAAG– 3’ R: 5’–CCCAAATCTGTCTTTCAGGC– 3’	0.200.20	0.50
G2-Mluc	F: [FAM] 5’–TGAAAAGAACTGGAGAGGCTTT– 3’ R: 5’–AGATTGATGAATGTGAAAGGTCAG– 3’	0.200.20	0.71
G6-Mluc	F: [PET] 5’–GGCTTTTTGAAAAGACTGAGG– 3’ R: 5’–ACATCAGCCAGTTCCTGTTC– 3’	0.200.20	0.25
G31-Mluc	F: [FAM] 5’–GATCACCAATCATGTAAGGTTCAC– 3’ R: 5’–AAGTCAAGGCCAAGCAAGTC– 3’	0.200.20	0.68
Panel 2
B8-Mluc	F: [VIC] 5’–AAATACCTGAGTGAGAACATTTAGTGGAG– 3’ R: 5’–CTCATTAACTTCATTGGTAAGTGTTGTACC– 3’	0.400.40	0.50
C113	F: [FAM] 5’- ACCTCCCTGCCCTGCAC– 3’ R: 5’–GCAATGCTTCCTCCAAGTCC– 3’	0.200.20	0.79
D15-Mluc	F: [NED] 5’–AAATTCTTTCCCTCCAAAGTGG– 3’ R: 5’–GCACGCTCAGACTCCTTCC– 3’	0.200.20	0.66
F19-Mluc	F: [PET] 5’–TGTAGCTAGCCATGGAGAAGG– 3’ R: 5’–AAATGGTTACATTACAGAAAATGCTC– 3’	0.200.20	0.59
G30-Mluc	F: [PET] 5’–GGCATGAACATGGAGTGAGG– 3’ R: 5’–GCTAGAAGTTATGGTCAATGTTCCTG– 3’	0.200.20	0.71
H23-Mluc	F: [VIC] 5’–TTGTCTACTAGCATTTGTCCAGTG– 3’ R: 5’–ATAGCTATGTTGCCTAACCTATTTACTC– 3’	0.400.40	0.43

The table provides the primers used in a specific multiplex PCR. The letters in square brackets indicate the fluoro-dyes used in one of the two sequences of each primer pair.