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Photomicrographs showing representative histology of human skin angiosarcoma. (A) Hematoxylin and eosin (H&E) staining; (B) CD31 immunolocalization using a mouse monoclonal antibody (catalog no. M0823, Dako); (C) EphB4 immunolocalization using a rabbit monoclonal antibody (catalog no. #14960, Cell Signaling Technology). Assessment of EphB4 immunoreactivity in human skin angiosarcoma showed 42% of cases were positive; n = 24. Scale bars 50 μm.
