Figure 9. TPNs action on all PPL1 lobes.
(A) Schematic specifying PPL1 region focused on for calcium imaging, with CsChrimson activation of TPN2 or TPN3. (B) Average response (black line) and individual traces (gray, blue, and red lines) of PPL1 regions to red light (no Gal4 driver control, TPN2 activation, and TPN3 activation, respectively). Control animals show no responses to red light, while activation of TPN3 generally increases calcium signals in all PPL1, and activation of TPN2 generally results in small but reliable decreases in calcium signal. Pink bar indicates time of laser stimulation. Horizontal scale bar = 5 s. Vertical scale bar = 1 △F/F. (C) Average area under the curve. Compared to no Gal4 controls, TPN2 and TPN3 show significant decreases and increases in calcium signal, respectively. Error bars indicate mean +/-± SEM. n = 9–13. Wilcoxon rank-sum: *p<0.05, **p<0.01, ***p<0.001.