Table 1.
Genotypes of Experimental Flies
| Figure | Description | Genotype |
|---|---|---|
| 1A | GRN anatomy | Gr66a-Gal4/CyO; UAS-cd8-tdTomato/Gr64fLexA, LexAop-cd2-GFP |
| 1B | TPN1 anatomy | UAS-GFP/+; R30A08-Gal4/+ |
| 1C | TPN2 anatomy | UAS-GFP/+; VT57358-Gal4/+ |
| 1D | TPN3 anatomy | tub>Gal80>/X; UAS-GFP/+; C220-Gal4/hsFLP, MKRS |
| 1 – fs1A | TPN1 single | UAS-GFP/+; R30A08-Gal4/+ |
| 1 - fs1B | TPN1 dendrite/axon | +/CyO; R30A08-Gal4/UAS-DenMark, UAS-synaptotagmin-eGFP |
| 1 - fs1C & D | TPN2 mosaics | tub>Gal80>/X; UAS-GFP/+; VT57358-Gal4/ hsFLP, MKRS |
| 1 - fs1E | TPN2 dendrite/axon | +/CyO; VT57358-Gal4/UAS-DenMark, UAS-synaptotagmin-eGFP |
| 1 - fs1F | TPN3 – 2nd Gal4 | UAS-GFP/+; C220-Gal4/+ |
| 1 - fs1G | TPN3 – 1st Gal4 | UAS-GFP/+; R11H09-Gal4/+ |
| 1 - fs1H | TPN3 – 2 cells only | UAS-GFP/UAS-RedStinger; C220-Gal4/R11H09-Gal4 |
| 1 - fs1I | TPN3 dendrite/axon | +/CyO; C220-Gal4/UAS-DenMark, UAS-synaptotagmin-eGFP |
| 2A & 2 – fs1 |
Sugar GRN doubles | UAS-cd8tdTomato/CyO; R30A08-Gal4 / Gr64fLexA, LexAop-cd2-GFP UAS-cd8tdTomato/CyO; VT57358-Gal4 / Gr64fLexA, LexAop-cd2-GFP UAS-cd8tdTomato/CyO; C220-Gal4 / Gr64fLexA, LexAop-cd2-GFP |
| 2A & 2 – fs1 |
Bitter GRN doubles | UAS-cd8tdTomato/Gr66a-LexA; R30A08-Gal4 / LexAop-cd2-GFP UAS-cd8tdTomato/Gr66a-LexA; VT57358-Gal4 / LexAop-cd2-GFP UAS-cd8tdTomato/Gr66a-LexA; C220-Gal4 / LexAop-cd2-GFP |
| 2A | Pheromone GRN doubles | UAS-cd8tdTomato/ppk23-LexA; R30A08-Gal4 / LexAop-cd2-GFP UAS-cd8tdTomato/ppk23-LexA; VT57358-Gal4 / LexAop-cd2-GFP UAS-cd8tdTomato/ppk23-LexA; C220-Gal4 / LexAop-cd2-GFP |
| 2A | Water GRN doubles | UAS-cd8tdTomato/ppk28-LexA, LexAop-cd2-GFP; R30A08-Gal4 / TM2 or 6b UAS-cd8tdTomato/ppk28-LexA, LexAop-cd2-GFP; VT57358-Gal4 / TM2 or 6b UAS-cd8tdTomato/ppk28-LexA, LexAop-cd2-GFP; C220-Gal4 / TM2 or 6b |
| 2B | Sugar GRN GRASP | Gr5a-LexA/X; UAS-cd8tdTomato/LexAop-CD4::spGFP11; R30A08-Gal4 or VT57358-Gal4 or C220-Gal4/UAS-CD4::spGFP1-10 |
| 2B | Bitter GRN GRASP | Gr66a-LexA/X; UAS-cd8tdTomato/LexAop-CD4::spGFP11; R30A08-Gal4 or VT57358-Gal4 or C220-Gal4/UAS-CD4::spGFP1-10 |
| 3 A-F top | TPN1 GCaMP | UAS-cd8tdTomato/UAS-GCaMP6s; R30A08-Gal4/UAS-GCaMP6s |
| 3 A-F middle | TPN2 GCaMP | UAS-cd8tdTomato/UAS-GCaMP6s; VT57358-Gal4/UAS-GCaMP6s |
| 3 A-E bottom and G | TPN3 GCaMP | UAS-cd8tdTomato/UAS-GCaMP6s; R11H09-Gal4/UAS-GCaMP6s |
| 3 – fs1 A | TPN1-GCaMP sugar-TRP | Gr5a-LexA/X; UAS-GCaMP5/UAS-cd8tdTomato; R30A08-Gal4/LexAop-dTRP |
| TPN1-GCaMP bitter-TRP |
Gr66a-LexA/X; UAS-GCaMP5/UAS-cd8tdTomato; R30A08-Gal4 /LexAop-dTRP |
|
| 3 – fs1 B | TPN2-GCaMP sugar-TRP | Gr5a-LexA/X; UAS-GCaMP5/UAS-cd8tdTomato; VT57358-Gal4 /LexAop-dTRP |
| TPN2-GCaMP bitter-TRP |
Gr66a-LexA/X; UAS-GCaMP5/UAS-cd8tdTomato; VT57358-Gal4 /LexAop-dTRP |
|
| 3 – fs1 C | TPN3-GCaMP sugar-TRP | Gr5a-LexA/X; UAS-GCaMP5/UAS-cd8tdTomato; C220-Gal4/LexAop-dTRP |
| TPN3-GCaMP bitter-TRP |
Gr66a-LexA/X; UAS-GCaMP5/UAS-cd8tdTomato; C220-Gal4/LexAop-dTRP |
|
| 4A | TPN2 & MB | MB-dsRed/UAS-GFP; VT57358-Gal4/ TM2 or 6b |
| 4F | TPN3 & MB | MB-dsRed/UAS-GFP; R11H09-Gal4/TM2 or 6b |
| 4 B-E | TPN2 & olfactory PN | GH146-QUAS, QUAS-mtdTomato / UAS-GFP; VT57358-Gal4/+ |
| 4 G-J | TPN3 & olfactory PN | GH146-QUAS, QUAS-mtdTomato / UAS-GFP; R11H09-Gal4/+ |
| 5 A & B | PER: noGal4 | UAS-CsChRimson/X; +; + |
| 5 A & B | PER: GR Gal4s | UAS-CsChRimson/X; Gr64f-Gal4/+; TM2 or TM6b/+ UAS-CsChRimson/X; Gr66a-Gal4/+; TM2 or TM6b/+ |
| 5 A & B | PER: TPN Gal4s | UAS-CsChRimson/X; +; R30A08-Gal4/+ UAS-CsChRimson/X; +; VT57358-Gal4/+ UAS-CsChRimson/X; +; R11H09-Gal4/+ |
| 5 C & D | Shi PER behavior | + / UAS- Shibire(ts) R30A08-Gal4/UAS-Shibire(ts) VT57358-Gal4/UAS-Shibire(ts) R30A08-Gal4, VT57358-Gal4/UAS-Shibire(ts) R11H09-Gal4/UAS-Shibire(ts) C220-Gal4/UAS-Shibire(ts) |
| 5 – fs1A & B | Gr-Gal4s | Gr64f-Gal4/+; TM2 or TM6b/+ Gr66a-Gal4/+; TM2 or TM6b/+ |
| 5 – fs1A & B | TPN-Gal4s | R30A08-Gal4/+ VT57358-Gal4/+ R11H09-Gal4/+ C220-Gal4/+ |
| 5 – fs1 C & D | TPN-Gal4s (controls for PER with Shi) | R30A08-Gal4/+ VT57358-Gal4/+ R11H09-Gal4/+ C220-Gal4/+ |
| 6B | Memory – TPN1 Shi | R30A08-Gal4/UAS-Shibire(ts) |
| 6C | Memory – TPN2 Shi | VT57358-Gal4/UAS-Shibire(ts) |
| 6D | Memory – TPN3 Shi | R11H09-Gal4/UAS-Shibire(ts) |
| 6F | Memory – TPN3 Chrimson | UAS-CsChRimson/X; +; R11H09-Gal4/+ |
| 6 – fs1A | TPN3 – 2nd Gal4 | X; +; C220-Gal4/UAS-Shibire(ts) |
| 6 – fs1B | TPN3 – 2nd Gal4 | UAS-CsChRimson/X; +; C220-Gal4/+ |
| 7 A & B | Memory – TPN2 Shi | VT57358-Gal4/UAS-Shibire(ts) |
| 7 C & D | Memory – TPN3 Shi | R11H09-Gal4/UAS-Shibire(ts) |
| 8 & 9 | PPL1 - control | UAS-CsChRimson/X; LexAop-GCaMP6s; TH-LexA/TM3-ser |
| 8 & 9 | PPL1 – TPN2 | UAS-CsChRimson/X; LexAop-GCaMP6s; TH-LexA/ VT57358-Gal4 |
| 8 & 9 | PPL1 – TPN3 | UAS-CsChRimson/X; LexAop-GCaMP6s; TH-LexA/ R11H09-Gal4 |
| 8 – fs1A | PPL1 imaging | UAS-CsChRimson/X; LexAop-GCaMP6s; TH-LexA/C220-Gal4 |
| 8 – fs1B | PPL1 imaging | UAS-CsChRimson/X; LexAop-GCaMP6s; TH-LexA/TM3-ser UAS-CsChRimson/X; LexAop-GCaMP6s; TH-LexA/C220-Gal4 |
| 8 – fs1C-E | PPL1 ex vivo imaging | UAS-CsChRimson/X; LexAop-GCaMP6s; TH-LexA/ VT57358-Gal4 UAS-CsChRimson/X; LexAop-GCaMP6s; TH-LexA/ R11H09-Gal4 |
| 10 | Gr66a-LexA, LexAop-CsChrimson / UAS-CD8-tdTomato; UAS-GCaMP6s / MB065B-Gal4 AD; R11H09-Gal4, UAS-GCaMP6s / MB065B-Gal4 DBD |