Table 1.
Overview of the metabolic pathways included in the metabolic reconstructions.
| Number of reactions in pathway
|
Number of reactions predicted essential*
|
||||||
|---|---|---|---|---|---|---|---|
| Superpathway | Metabolic pathway | L. loa | Ov and wOv | wOv only | L. loa | Ov and wOv | wOv only |
| Amino acid metabolism | Alanine, aspartate and glutamate metabolism | 14 | 15 | 3 | 1 | 1 | 0 |
| Arginine and proline metabolism | 26 | 25 | 2 | 3 | 3 | 0 | |
| Biosynthesis of amino acids | 37 | 41 | 9 | 3 | 3 | 0 | |
| Cysteine and methionine metabolism | 17 | 19 | 2 | 4 | 4 | 0 | |
| Glycine, serine and threonine metabolism | 17 | 17 | 3 | 1 | 1 | 0 | |
| Biosynthesis of other secondary metabolites | Streptomycin biosynthesis | 4 | 4 | 0 | 2 | 2 | 0 |
| Carbohydrate metabolism | Amino sugar and nucleotide sugar metabolism | 23 | 23 | 3 | 4 | 4 | 0 |
| Butanoate metabolism | 8 | 8 | 0 | 1 | 0 | 0 | |
| Inositol phosphate metabolism | 12 | 12 | 0 | 3 | 3 | 0 | |
| Pentose phosphate pathway | 17 | 18 | 0 | 2 | 2 | 0 | |
| Propanoate metabolism | 14 | 14 | 1 | 1 | 1 | 0 | |
| Pyruvate metabolism | 20 | 20 | 1 | 1 | 1 | 0 | |
| Energy metabolism | Methane metabolism | 16 | 16 | 0 | 2 | 2 | 0 |
| Lipid metabolism | Arachidonic acid metabolism | 16 | 14 | 0 | 3 | 3 | 0 |
| Biosynthesis of unsaturated fatty acids | 14 | 15 | 2 | 7 | 2 | 0 | |
| Fatty acid biosynthesis | 3 | 15 | 32 | 1 | 1 | 0 | |
| Fatty acid degradation | 32 | 32 | 0 | 19 | 0 | 0 | |
| Fatty acid elongation | 28 | 28 | 0 | 24 | 0 | 0 | |
| Fatty acid metabolism | 48 | 57 | 32 | 31 | 2 | 0 | |
| Glycerolipid metabolism | 9 | 9 | 0 | 2 | 2 | 0 | |
| Glycerophospholipid metabolism | 22 | 22 | 1 | 6 | 6 | 0 | |
| Sphingolipid metabolism | 16 | 16 | 0 | 5 | 5 | 0 | |
| Metabolism of cofactors and vitamins | Nicotinate and nicotinamide metabolism† | 11 | 12 | 1 | 1 | 0 | 1 |
| One carbon pool by folate | 10 | 10 | 6 | 1 | 0 | 0 | |
| Pantothenate and CoA biosynthesis | 11 | 11 | 1 | 3 | 3 | 0 | |
| Metabolism of other amino acids | Beta-alanine metabolism | 9 | 9 | 0 | 1 | 1 | 0 |
| Glutathione metabolism | 19 | 18 | 0 | 2 | 2 | 0 | |
| Metabolism of terpenoids and polyketides | Terpenoid backbone biosynthesis | 12 | 12 | 2 | 8 | 8 | 0 |
| Tetracycline biosynthesis | 1 | 1 | 0 | 1 | 1 | 0 | |
| Nucleotide metabolism | Purine metabolism | 53 | 63 | 9 | 7 | 3 | 0 |
| Pyrimidine metabolism | 47 | 51 | 7 | 7 | 5 | 0 | |
| Transport reactions | Extracellular transport | 43 | 43 | 0 | 23 | 18‡ | 0 |
| Total (non-redundant) number of reactions in pathways | 428 | 455 | 72 | 86 | 49 | 1 | |
| Total number of reactions in reconstructions | 777 | 796 | 100 | 112 | 70 | 1 | |
One reaction was essential either from L. loa (with or without genetic evidence); O. volvulus (Ov) and Wolbachia (wOv) with reactions in the O. volvulus reconstruction (both from gene annotation and added in pathway gap-filling) not being contributed by wOv only; or wOv with reactions in the Ov reconstruction supported by genetic evidence only from wOv.
Only a single reaction, in the nicotinate and nicotinamide pathway, is essential and uniquely provided by wOv. Transport reactions for both nematode and Wolbachia lack genetic evidence therefore making wOv only and O. volvulus and wOv transporters indistinguishable.
Note that of the 18 transport reactions essential to O. volvulus, all are essential to L. loa as well except for threonine transport. Also reported is the total number of reactions belonging to the pathways listed here, as well as the total number of reactions in the reconstructions.