Skip to main content
. 2017 Feb 15;12(2):e0172130. doi: 10.1371/journal.pone.0172130

Fig 2. ABCA3/Ph-TOR staining in HPAEpiC and relative expression of KRT14/SFTPA.

Fig 2

A) Immunocytochemistry staining in human HPAEpiC, showing that, at T = 0, the cells are ABCA3 and Ph-TOR positive. B) The mRNA expression of genes of interest, KRT14 and Surfactant protein A1 (SFTPA1) (type 2 pneumocyte marker), was monitored just after thawing (T0), after 7 hours (T7), and after 48 hours (T48). The normalization was carried out by using GAPDH and RPL13a housekeeping genes; data are reported as mean ± SD, n = 3, *p<0.05 compared to T0.