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. 2017 Feb 15;12(2):e0169011. doi: 10.1371/journal.pone.0169011

Fig 5. Sensitivity and specificity of the WbLDR colorimetric NINA-LAMP assay using Bst 2.0 WS.

Fig 5

(A) LAMP reactions incubated in the NINA heater exhibit the same sensitivity as those incubated in a qPCR machine with DNA amplification monitored in real time using a dsDNA binding dye. Genomic W. bancrofti DNA dilutions equivalent to 1/5 mf (1), 1/50 mf (2), 1/500 mf (3), 1/5000 mf (4), 1/50,000 mf (5), 1/500,000 mf (6), 1/5,000,000 mf (7) and H2O (8) were used as template. The end point color change of the LAMP reactions incubated in the NINA heater are shown in the upper panel. Real time amplification curves and end point color change of LAMP reactions are shown in the lower panel. (B) Species-specificity using 100 pg of various genomic DNAs as template. (C) Detection of W. bancrofti DNA in blood samples from 8 different LF positive patients (S1-S8). Species names are abbreviated as follows: W. bancrofti (Wb), B. malayi (Bm), O. volvulus (Ov), Aedes aegypti (Aa) and Homo sapiens (Hs). All experiments were performed in triplicate. One representative of each is shown.