Skip to main content
. 2017 Feb 15;12(2):e0171857. doi: 10.1371/journal.pone.0171857

Fig 5. Osteoblast recruitment in the flow direction and motility under shear are increased with ROCK interference.

Fig 5

(a) Osteoblasts with ROCK-shRNA (ROCK-sh) under FF25 showed significantly greater number of cells migrating with the flow direction. (b) Even after the initial period, the speeds of ROCK-silenced cells were greater for both static and sheared conditions (e.g., 60 min). (c) The displacement of ROCK-shRNA FF25 group assessed after 120 min was significantly greater compared with other conditions. (d) The ROCK-shRNA static group was less confined in migration path. (e) The ROCK-shRNA cells paused significantly less during the migration. (f) The RMS displacement shows that the collective migration of the ROCK-shRNA FF25 group was continued throughout the measurement time resulting in greater RMS displacement at 120 min. The dashed vertical line marks 30 min after the flow onset. *: comparison with vector control static. ‡: comparison with vector control FF25. +: comparison with ROCK-shRNA static. Single, double, and triple symbols represent p < 0.05, 0.01, and 0.001, respectively.