Fig 5. Characterization of sRNA-producing genes.

(A) Number of genes that produce sRNAs depending on F. graminearum strains. (B) Number of genes that produce sRNAs with 5′-U depending on F. graminearum strains. (C) Correlation analyses of transcriptomes of Fgdicer1 Fgdicer2 and Fgago1 Fgago2 compared to that of the wild type depending on sRNA counts. The log₂ ratio of transcript abundance in Fgdicer1 Fgdicer2 versus wild-type (x axis) and Fgago1 Fgago2 versus wild-type (y axis) is plotted. Colors indicate the sRNA density (reads per kilobase). (D) Correlation analyses of sRNA counts and transcript abundance. Gene numbers with corresponding log₂ ratio of transcript abundance in Fgdicer1 Fgdicer2 or Fgago1 Fgago2 versus the wild-type strain Z-3639 were counted. Most genes producing antisense sRNAs more than 1000 counts per kilobase (red graphs) were positively regulated in Fgdicer1 Fgdicer2 and Fgago1 Fgago2 compared to those in the wild type. Colors indicate the sRNA density denoted in Fig 5C.