Table 1. Screening results, including compound totals for each library.

	Bioactives	GHCDL
Total compounds	10,453	68,489
Hits (≥ 70% inhibition)	734	78
Raw hit rate (%)	7.02	0.11
Cytotoxicity filter	130	N/A
Filtered hit rate (%)	1.24	0.11
Reconfirmed hits	75	31
Reconfirmation rate (%)	57.7	39.7
EC50 ≤ 1 μM	49	18
Selected filtered hits (CC50:EC50 ≥ 10)	10	2

A host cell cytotoxicity filter was applied (≥ 40% HCT-8 cell inhibition) and the remaining compounds were tested in triplicate for reconfirmation. Reconfirmed hits were then tested in dose-response, and compounds were further filtered by potency and selectivity (final row).