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. Author manuscript; available in PMC: 2017 Feb 15.
Published in final edited form as: Leuk Res. 2013 Sep 29;37(12):1719–1725. doi: 10.1016/j.leukres.2013.09.019

Fig. 3.

Fig. 3

Analyses of survivin protein and mRNA levels. WSU-CLL cells were exposed to 2.0 μM As2O3 for 0, 24, 48 and 72 h. Survivin was analyzed by Western blotting. (A) Representative immunoblots of the expression level of survivin in cells treated with 2.0 μM As2O3 for 0, 24, 48 and 72 h. (B) β-actin expression was used as a loading control, and the relative survivin protein levels (means ± SD, n = 3) were determined. Survivin mRNA levels were measured by RT-PCR. (C) RT-PCR images of survivin in WSU-CLL cells treated with As2O3 for different lengths of time. (D) Quantification of the survivin mRNA level in WSU-CLL cells treated with As2O3 for different lengths of time in three separate experiments. As2O3 reduced survivin transcriptional activity.