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Fibroblast cytoskeletal response to tissue stretch ex vivo. Tissue explants from Control and sclerodermatous graft-versus-host disease mice were excised immediately after euthanasia at week 2 or week 4, then stretched (S) versus non-stretched ex vivo for 2 h, followed by fixation and staining with phalloidin for measurement of cell body cross-sectional area (outlined as described in Section “Materials and Methods”).
