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. 2017 Feb 16;8:71. doi: 10.3389/fphys.2017.00071

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Copyright © 2017 Chng, Ong, Ching, Chen, Hiong, Wong, Chew, Lam and Ip.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Absolute quantification of mRNA (×10² copies of transcripts per ng total RNA) of urea transporter a2a (ut-a2a) in the gills of Protopterus annectens kept in fresh water on day 0 (FW; control), after 6 days (d; induction phase), or 6 months (mon; maintenance phase) of aestivation, or after 1 or 3 d of arousal (Ar) from 6 mon of aestivation. Results represent means ± S.E.M (N = 4). Means not sharing the same letter are significantly different (P < 0.05).