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. 2017 Feb 16;8:71. doi: 10.3389/fphys.2017.00071

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Copyright © 2017 Chng, Ong, Ching, Chen, Hiong, Wong, Chew, Lam and Ip.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Protein abundance of urea transporter a2b (Ut-a2b) in the gills of Protopterus annectens kept in fresh water on day 0 (FW; control), after 6 days (d; induction phase) or 6 months (mon; maintenance phase) of aestivation, or after 1 or 3 d of arousal (Ar) from 6 mon of aestivation. (A) An example of immunoblot of Ut-a2b (left) and Ut-a2b pre-incubated with immunising peptide for the peptide competition assay (PCA; right). (B) The protein abundance of Ut-a2b expressed as arbitrary densitometric units per 200 μg protein. Results represent mean ± S.E.M. (N = 3). Means not sharing the same letter are significantly different (P < 0.05).