Table 1.

Primers used for PCR, RACE, and qPCR of urea transporter a2a (ut-a2a) and urea transporter a2b (ut-a2b) from the gills of Protopterus annectens.

Gene	Primer type	Primer sequence (5′to 3′)
ut-a2a	PCR	Forward: AGGAACTGAAGTCACTCATGG
		Reverse: GGAAGACTGCAATGATATTGTG
	5′-RACE	TTAGCTTCTGGGTATGTGACTT
	3′-RACE	GGAGACATGGAAGAATTTGG
	qPCR	Forward: AAAGAATCTGTGAAGCCAGTAG
		Reverse: ATCACTTGTGCTGTACCTCTTA
ut-a2b	PCR	Forward: AACAGTTCGTAAAGTCATAGCC
		Reverse: CACAGAATCATTGTTGTCTTCC
	5′-RACE	CCACTGCACTTGTCAGAATAGGG
	3′-RACE	CAACTCTGATTCAGCCGCAGACCT
	qPCR	Forward: CTAACACATTTCCGATGACAG
		Reverse: CCTCCTCAGTAGATTGTTCAG