Table 1.
Circulating microRNAs (miRNAs) profiled in selected human tuberculosis studies.
| Platform/assay | Biological fluid | Notable miRNAs detected (arrows indicate direction of expression) | Data normalization | Reference |
|---|---|---|---|---|
| miRCURY LNA array (Exiqon) | Serum and sputum | miR-93*↑, miR-29a↑ | Median normalization | Fu et al. (147) |
| Gene Amp PCR system 9700 (Applied Biosystems) | U6 snRNA | |||
| miRCURY LNA array (Exiqon) | Sputum | miR-3179↑, miR-147↑, miR-19b-2*↓, miR-29a↑ | Median normalization | Yi et al. (148) |
| GeneAmp PCR System 9700 (Applied Biosystems) | U6 snRNA | |||
| TaqMan Low Density array (Applied Biosystems) | Serum | miR-361-5p↑, miR-889↑, miR-576-3p↑ | cel-miR-238 | Qi et al. (149) |
| TaqMan RT-qPCR (Applied Biosystems) | miR-16 | |||
| 7500 Real-Time PCR system (Applied Biosystems) | Serum | miR-197↑ | SNORD68 | Abd-El-Fattah et al. (150) |
| Solexa Small RNA-seq (Illumina) | Serum | miR-378↑, miR-483-5p↑, miR-22↑, miR-29c↑, miR-101↓, miR-320b↓ | No information provided concerning miRNA-sequencing normalization method | Zhang et al. (151) |
| SYBR green RT-qPCR assay | miR-16 | |||
| Solexa Small RNA-seq (Illumina) | Serum | miR-516b↑, miR-486-5p↓, miR-196b↑, miR-376c↑ | Total copy number of each sample was normalized to 100,000 | Zhang et al. (152) |
| TaqMan RT-qPCR (Applied Biosystems) | cel-miR-238 | |||
| SYBR green RT-qPCR assay | Serum | miR-155↓ | U6 snRNA | Zhang et al. (154) |