Table 2.
Circulating microRNAs (miRNAs) profiled in selected sepsis studies.
| Platform/assay | Biological fluid | Notable miRNAs detected (arrows indicate direction of expression) | Data normalization | Reference |
|---|---|---|---|---|
| miRNA microarray (Agilent Technologies) | Plasma | miR-486↑, miR-182↑, miR-150↓, miR-342-5p↓ | Median normalization | Vasilescu et al. (158) |
| RT-qPCR TaqMan MicroRNA assays (Applied Biosystems) | U6B snRNA | |||
| RT-qPCR TaqMan MicroRNA assays (Applied Biosystems) | Plasma | miR-150↑, miR-146a NS, miR-223 NS | cel-miR-39 | Puskarich et al. (159) |
| RT-qPCR miScript system (Qiagen) | Serum | miR-146a↓, miR-223↓ | mmu-miR-295 | Wang et al. (161) |
| RT-qPCR miScript system (Qiagen) | Serum | miR-133a↑ | SV40 | Tacke et al. (164) |
| RT-qPCR miScript system (Qiagen) | Serum | miR-122↑ | SV40 | Roderburg et al. (165) |
| GeneChip miRNA 1.0 arrays (Affymetrix) | Serum | miR-297↑, miR-574-5p↓ | 5S rRNA | Wang et al. (166) |
| RT-qPCR miRcute (Tiangen Biotech Company) | ||||
| Solexa Small RNA-seq (Illumina) | Serum | miR-193b*↑, miR-15↑, miR-122↑, miR-483-5p↑, miR-16↓, miR-223↓ | U6 snRNA | Wang et al. (167) |
| RT-qPCR TaqMan MicroRNA assays (Applied Biosystems) |