Table 3.
Circulating microRNAs (miRNAs) profiled in selected hepatitis B studies.
| Platform/assay | Biological fluid | Notable miRNAs detected (arrows indicate direction of expression) | Data normalization | Reference |
|---|---|---|---|---|
| RT-qPCR TaqMan MicroRNA assays (Applied Biosystems) | Serum | miR-122↑, miR-16↑, miR-223↑, miR-19b↑, miR-20a↑, miR-92a↑, miR-106a↑, let-7b↑, miR-194↑ | U6 snRNA | Ji et al. (170) |
| RT-qPCR TaqMan MicroRNA assays (Applied Biosystems) | Serum | miR-210↑ | cel-miR-39 | Song et al. (171) |
| RT-qPCR miRcute (Tiangen Biotech Company) | Serum | miR-124↑ | 5S rRNA | Wang et al. (172) |
| miRNA microarray (Agilent Technologies) | Plasma | miR-4695-5p↑, miR-486-3p↓, miR-497-5p↓ | Quantile normalization | Zhang et al. (173) |
| SYBR Green I-based RT-qPCR with individual miRNA-specific primers (Applied Biosystems) | Plasma | miR-122↑ | U6 snRNA | Zhang et al. (181) |
| Thunderbird SYBR qPCR mix (Toyobo, Japan) | Plasma | miR-122↑ | No information provided | Zhang et al. (182) |
| SYBR Green PCR Master Mixture (Takara) | Serum | miR-122↑ | miR-181a | Xu et al. (183) |
| RT-qPCR TaqMan MicroRNA assays (Applied Biosystems) | Serum | miR-122↑ | No information provided | Waidmann et al. (186) |
| RT-qPCR miRNA arrays and individual assays (Exiqon) | Plasma | miR-122-5p↑, miR-122-3p↑, miR-99a-5p↑, miR-125b-5p↑ | Global mean normalization, U6, and geometric mean normalization | Winther et al. (187) |
| TaqMan probe-based RT-qPCR (Applied Biosystems) | Serum | miR-122↑, miR-let7c↑, miR-23b↑, miR-150↑ | Plant MIR-168 | Chen et al. (189) |
| Solexa Small RNA-seq (Illumina) | Serum | miR-375↑, miR-10a↑, miR-223↑, miR-423↑ | Plant MIR-168 | Li et al. (190) |
| TaqMan probe-based RT-qPCR (Applied Biosystems) |