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. 2016 Oct 3;53(2):167–178. doi: 10.1007/s11626-016-0096-z

Figure 1.

Figure 1.

Zscan4 mRNA expression in the testis and ovary. (A) Tissue specificity of mRNA expression for the indicated genes was analyzed by RT-PCR. E16 fetal ovary at embryonic day 16; 4w 4-wk ovary; Em− and Em+ Emerald GFP negative and positive populations of mouse MC1 ES cells enriched by FACS, respectively; Syce1 and Sycp3: meiosis specific markers. MuERV-L retrotransposon supposed to be highly expressed in Em+ ES cell. (B) Expression of endogenous Zscan4 and exogenous Emerald GFP, which is knocked-in at the Zscan4c locus, was analyzed by RT-PCR. RNA was extracted from the isolated oocytes and testis in Emerald GFP knock-in (Z4EmKI) and wild-type mice and from Z4EmKI ES cells. Schematic Z4EmKI locus is shown (upper). Em− and Em+ Emerald negative and positive enriched populations of Z4EmKI ES cells, respectively. Meikin meiosis I specific marker (Kim et al. 2015). RT− control PCR without reverse transcription.