Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Oct 3;53(2):167–178. doi: 10.1007/s11626-016-0096-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2016

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

PMC Copyright notice

Figure 4. — Zscan4 localization in SN- and NSN-type GV oocytes. (A) GV oocytes, classified as SN type and NSN type by heterochromatin morphology relative to nucleolus (shown by asterisk), were immunostained as indicated. The spatial localization pattern of Zscan4 was classified as spotty (limited number of foci) and diffusive (dispersed in nuclei). The immunostaining pattern of Zscan4 is quantified in the right graph. The number of analyzed GV oocytes (pooled from four independent experiments) is indicated. **P < 0.001 (Pearson’s chi-square test). (B) Intensity profile of Zscan4 immunostaining signal obtained with the FluoView Software from representative GV oocytes with a SN spotty (top, n = 21), SN diffuse (middle, n = 11), and NSN (bottom, n = 23). The value of pixel intensity on Z-axis is equal to 200 for the spotty signal (SN spotty) and to 180 (SN diffuse, NSN) for the oocytes with diffuse signal as inferable from the picture. Corrected total cell fluorescence (CTCF, arbitrary units) is shown in the bottom graph with SEM. C Zscan4 and DAPI signals were scanned across a nucleolus in SN-type GV oocyte (dashed line). Signal intensity of DAPI (green) and Zscan4 (gray bar) is shown on the bottom. D SN-type and NSN-type GV oocytes were immunostained for KAP1: a heterochromatic protein which is potentially associated with Zscan4. E SN-type GV oocyte was immunostained for anti-centromere antigen (ACA). Scale bars, 10 μm.