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. 2016 Oct 3;53(2):167–178. doi: 10.1007/s11626-016-0096-z

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© The Author(s) 2016

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Figure 5. — Localization patterns of Zscan4 correlate with Pol II-mediated transcription activity in GV oocytes. (A) SN- and NSN-type GV oocytes were immunostained for Pol2(S2P): RNA polymerase II CTD repeat (phospholylated Ser2), a marker for active transcriptional elongation. Weak Pol2(S2P) in Zscan4-spotty SN-type oocytes(top, n = 27/27), intense Pol2(S2P) in Zscan4-diffusive SN-type oocytes (middle, n = 2/2), intense Pol2(S2P) in Zscan4-diffusive NSN-type oocytes (bottom, n = 3/3). (B) SN- and NSN-type GV oocytes were immunostained for Pol2(S5P): RNA polymerase II CTD repeat (phospholylated Ser5), a marker for active transcriptional initiation.