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. 2017 Feb 14;23(6):964–975. doi: 10.3748/wjg.v23.i6.964

Figure 5.

Figure 5

Enhanced recovery of long-term cryopreserved intestinal organoids was dependent on the timing of Y-27632 treatment. Undissociated organoids (P3) were treated with 10 μmol/L Y-27632 at the indicated time points. A: Representative morphology of organoids cultured on day 7 in the presence of ENR medium after thawing organoids cryopreserved for 3 mo in the presence of 10% DMSO. Scale bars, 200 μm. B: Quantification of recovery from cryopreserved organoids was performed using MTT assays. The data are shown as means ± SDs of triplicate experiments (bP < 0.01, two-way analysis of variance with Dunnett’s T3 tests) and normalized to untreated samples. MTT: Methyl thiazolyl tetrazolium; ENR: Epidermal growth factor/Noggin/R-spondin1.